Publications

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Research Highlights

Messenger, S.R., McGuinniety, E.M.R., Stevenson, L.J., Owen, J.G., Challis, G.L., Ackerley, D.F. & Calcott, M.J. (2024). Metagenomic domain substitution for the high-throughput modification of nonribosomal peptides. Nature Chemical Biology. 2. 251-260

Rich MH, Sharrock AV, Mulligan TS, Matthews F, Brown AS, Lee-Harwood HR, Williams EM, Copp JN, Little RF, Francis JJB, Horvat CN, Stevenson LJ, Owen JG, Saxena MT, Mumm JS, Ackerley DF. (2023). A metagenomic library cloning strategy that promotes high-level expression of captured genes to enable efficient functional screening. Cell Chemical Biology. 30(12). 1680-1691

Ganley M., Holz L.E., Minnell J.J., de Menezes M.N., Burn O.K., Poa K.C.Y., Draper S.L., English K., Chan S.T.S., Anderson R.J., Compton B.J., Marshall A.J., Cozijnsen A., Chua Y.C., Ge Z., Farrand K.J., Mamum J.C., Xu C., Cockburn I.A., Yui K, Bertolino P., Gras S., Le Nours J., Rossjohn J., Fernandez-Ruiz D., McFadden G.I., Ackerley D.F., Painter G.F., Hermans I.F. & Heath W.R. (2023). mRNA vaccine against malaria tailored for liver-resident memory T cells. Nature Immunology. 24(9). 1487-1498

Sharrock AV*, Mulligan TS*, Hall KR, Williams EM, White DT, Zhang L, Emmerich K, Matthews F, Nimmagadda S, Washington S, Le KD, Meir-Levi D, Cox OL, Saxena MT, Calof AL, Lopez-Burks ME, Lander AD, Ding D, Ji H, Ackerley DF*, Mumm JS*. (2022). NTR 2.0: a rationally engineered prodrug-converting enzyme with substantially enhanced efficacy for targeted cell ablation. Nature Methods. 19. 205-215

Calcott, M. J., Owen, J. G., & Ackerley, D. F. (2020). Efficient rational modification of non-ribosomal peptides by adenylation domain substitution. Nature Communications. 11(1). 4554

Copp, J. N., Pletzer, D., Brown, A. S., Van der Heijden, J., Miton, C. M., Edgar, R. J., Rich, M. H., Little, R. F., Williams, E. M., Hancock, R., Tokuriki, N., & Ackerley, D. F. (2020). Mechanistic Understanding Enables the Rational Design of Salicylanilide Combination Therapies for Gram-Negative Infections. mBio. 11(5). e02068-20

Hall, K. R., Robins, K. J., Williams, E. M., Rich, M. H., Calcott, M. J., Copp, J. N., Little, R. F., Schwörer, R., Evans, G. B., Patrick, W. M. & Ackerley, D. F. (2020). Intracellular complexities of acquiring a new enzymatic function revealed by mass-randomisation of active-site residues. eLife. 9. e59081

Zhang, L., Chen, C., Fu,J., Lilley, B., Berlinicke, C., Hansen, B., Ding, D., Wang, G., Wang, T., Shou, D., Ye, Y., Mulligan, T., Emmerich, K., Saxena, M., Hall, K., Sharrock, A., Brandon, C., Park, H., Kam, T., Dawson, V., Dawson, T., Shim, J., Hanes, J., Ji, H., Liu, J., Qian, J., Ackerley, D., Rohrer, B., Zack, D. & Mumm, J. (2021). Large-scale phenotypic drug screen identifies neuroprotectants in zebrafish and mouse models of retinitis pigmentosa. Elife. 10. e57245

ALL PUBLICATIONS

(2025)

Calcott ML, Kroger J & Ackerley DF. (2025). A rapid combinatorial assembly method for gene cluster characterisation illuminates glidobactin biosynthesis. Synthetic and Systems Biotechnology. 11. 85-90

Valiauga B, Žulpaitė D, Sharrock AV, Ackerley DF & Čėnas N. (2025). Novel TdsD nitroreductase: characterization of kinetics and substrate specificity. Biotechnology Letters. 47 (5). 103

Ramirez-Garcia JL, Lee-Harwood H, Ackerley DF, Kelly M, Matoto SV, Hunt P, Singh AJ & Keyzers RA. (2025). Purpuramine R, a New Bromotyrosine Isolated from Pseudoceratina cf. verrucosa Collected in the Kingdom of Tonga. Marine Drugs. 23(5). 186

Valiauga B, Skurr TW, Sharrock AV, Ackerley DF & Čėnas N. (2025). A minireview on oxygen-insensitive nitroreductases: reaction mechanisms and their biomedical impact. Chemija. 36 (3)

(2024)

Sharrock, A.V., Mumm, J.S., Williams, E.M., Čėnas, N., Smaill, J.B., Patterson, A.V.; Ackerley, D.F., Bagdžiūnas, G. & Arcus, V.L. (2024). Structural Evaluation of a Nitroreductase Engineered for Improved Activation of the 5-Nitroimidazole PET Probe SN33623. International Journal of Molecular Science. 25. 6593

Valiauga,B., Bagdžiūnas, G., Sharrock, A.V., Ackerley, D.F. & Čėnas, N. (2024). The Catalysis Mechanism of E. coli Nitroreductase A, a Candidate for Gene-Directed Prodrug Therapy: Potentiometric and Substrate Specificity Studies. International Journal of Molecular Sciences. 25 (8). 4413

Emmerich K, Hageter J, Hoang T, Lyu P, Sharrock AV, Ceisel A, Thierer J, Chunawala Z, Nimmagadda S, Palazzo I, Matthews F, Zhang L, White DT, Rodriguez C, Graziano G, Marcos P, May A, Mulligan T, Reibman B, Saxena MT, Ackerley DF, Qian J, Blackshaw S, Horstick E & Mumm JS. (2024). A large-scale CRISPR screen reveals context-specific genetic regulation of retinal ganglion cell regeneration. Development. 151 (15). dev202754

Messenger, S.R., Ackerley, D.F. & Calcott, M.J. (2024). Heterologous Expression of Epoxomicin in Escherichia coli. American Chemical Society. Epub ahead of print

Messenger, S.R., McGuinniety, E.M.R., Stevenson, L.J., Owen, J.G., Challis, G.L., Ackerley, D.F. & Calcott, M.J. (2024). Metagenomic domain substitution for the high-throughput modification of nonribosomal peptides. Nature Chemical Biology. 2. 251-260

(2023)

Sharrock, A.V., Mumm, J.S., Bagdžiūnas, G., Čėnas, N., Arcus, V.L., Ackerley, D.F. (2023). The Crystal Structure of Engineered Nitroreductase NTR 2.0 and Impact of F70A and F108Y Substitutions on Substrate Specificity. International Journal of Molecular Sciences. 24(7)

Brown, A.S., Owen, J.G. & Ackerley, D.F. (2023). Directed evolution of the BpsA carrier protein domain for recognition by non-cognate 4′-phosphopantetheinyl transferases to enable inhibitor screening. Methods in molecular biology (Clifton, N.J.). 2670. 145–163

Rich MH, Sharrock AV, Mulligan TS, Matthews F, Brown AS, Lee-Harwood HR, Williams EM, Copp JN, Little RF, Francis JJB, Horvat CN, Stevenson LJ, Owen JG, Saxena MT, Mumm JS, Ackerley DF. (2023). A metagenomic library cloning strategy that promotes high-level expression of captured genes to enable efficient functional screening. Cell Chemical Biology. 30(12). 1680-1691

Stevenson, L.J., Robins, K.J., Sharma, J.K., Williams, E.M., Sharrock, A.V., Williamson, A.K., Arcus, V.L., Patrick, W.M. & Ackerley, D.F.. (2023). Development of a compartmentalised self-replication protocol for selection of superior blunt-end DNA ligases. Enzyme and Microbial Technology. 163. 110153

Tucker, T.R., Knitter, C.A., Khoury, D.M., Eshghi, S., Tran, S., Sharrock, A.V., Wiles, T.J., Ackerley, D.F., Mumm, J.S. & Parsons, M.J. (2023). An inducible model of inducible chronic hyperglycemia. Disease Models & Mechanisms. Advance online publication. https://doi.org/10.1242/dmm.050215

Ganley M., Holz L.E., Minnell J.J., de Menezes M.N., Burn O.K., Poa K.C.Y., Draper S.L., English K., Chan S.T.S., Anderson R.J., Compton B.J., Marshall A.J., Cozijnsen A., Chua Y.C., Ge Z., Farrand K.J., Mamum J.C., Xu C., Cockburn I.A., Yui K, Bertolino P., Gras S., Le Nours J., Rossjohn J., Fernandez-Ruiz D., McFadden G.I., Ackerley D.F., Painter G.F., Hermans I.F. & Heath W.R. (2023). mRNA vaccine against malaria tailored for liver-resident memory T cells. Nature Immunology. 24(9). 1487-1498